(mouse/rat) EIA kit

Insulin Assays
Reference Insulin A05105

Insulin data

Stability 3 years
Storage -20°C
Shipping Dry ice
Specificity Rat Insulin: 100%
Hamster Insulin: 100%
Human Insulin: 100%
Mouse Insulin: 100%
Porcine Insulin: 100%
Sheep Insulin: 100%
Application media Plasma and serum
Pre-analytical phase No extraction required; inhibitor cocktail to prevent hemolysis consequences
Tracer label AChE®
Sample Volume 50 µL
Detection limit 0.08 ng/mL
Standard curve range 0.08-10 ng/mL
Correlated Keywords measures, kits, eias, enzyme, immunoassays, ELISAs, diabetes, spi-bio, spibio, A05105, adipokines, rat, insulin

Insulin description

Insulin is a polypeptide hormone with molecular weight of 6,000 Daltons, composed of two peptides chains, A and B, jointed by two cross-linked disulphide bonds and synthesised by the beta cells of the islets of Langherans of the pancreas. Insulin influences most of the metabolic functions of the body. Its best known action is to lower the blood glucose concentration by increasing the rate at which glucose is converted to glycogen in the liver and muscles and to fat in adipose tissue, by stimulating the rate of glucose metabolism and by depressing gluconeogenesis. Focus on hemolysis: Hemolysis interferes with the assay by degrading insulin. To prevent hemolysis consequences, SPI-BIO has an inhibitor cocktail and a procedure available.

Insulin documentation

Download insulin technical file

Insulin articles

Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and it use as a tracer or marquer in enzymo-immunological determinations. Grassi J. & Pradelles Ph. United States patent, N° 1,047,330. September 1 2, Synergistic activatin of glucose-6-phosphate dehydrogenase and NAD(P)H oxidase by Src kinase elevates superoxide in type 2 diabetic, Zucker fa/fa, rat liver. Rakhee S. Gupte1,2, Beverly C. Floyd2, Mark Kozicky2, Shimran George2, Zoltan I., Ungvari2, Vanessa Neito2, Michael S. Wolin2, and Sachin A Gupte1,2, * - 1 Department of biochemistry & moleculal biology, University of South Alabama, Mobile, AL 36688


Rat Insulin concentration